A strain of B. megaterium isolated from infant milk formula was determined to be non-cytotoxic, yet demonstrated adherence similar to that shown by a strain of Listeria monocytogenes as well as some invasion capability (Rowan et al. Providenti, M.A., Begin, M., Hynes, S., Lamarche, C., Chitty, D., Hahn, J., Beaudette, L.A., Scroggins, R., and Smith, M.L. Development of a two-step cultivation strategy for the production of vitamin B12 by Bacillus megaterium.
, The etymology listed in LPSN is, despite being not quite correct, a fusion of the first and third interpretation Gr. In one case, a non-hemolytic, non-motile Bacillus isolated from a blood culture, suspected to be B. anthracis, was in fact B. megaterium (Dib et al. LWT-Food Sci. Int B Bact Nomencl T 1, 35–36. Rev Argent Microbiol 42, 216-225. Bacillus megaterium CYP102A1 oxidation of acyl homoserine lactones and acyl homoserines. All the other Bacillus species in Group 2 have swollen sporangia. Verslyppe B., De Smet W., De Baets B., De Vos P., Dawyndt P. (2014). Syst Appl Microbiol 37, 42-50. Lett Appl Microbiol 13, 202-206. To update information about current uses, the Government launched a mandatory information-gathering survey under section 71 of CEPA, as published in the Canada Gazette, Part I, on October 3, 2009 (section 71 notice). Effects of Foliar Application of Plant Growth Promoting Bacterium on Chemical Contents, Yield and Growth of Tomato (Lycopersicon esculentum L.) and Cucumber (Cucumis sativus L.). J Invertebr Pathol 80, 7-12. Nguyen, M.T., Ranamukhaarachchi, S.L., and Hannaway, D.B. A strain of B. megaterium isolated from forest soil was able to solubilize iron, manganese and copper from phosphogypsum, a waste product of the production of fertilizer from phosphate rock (Ştefănescu et al.
Bordetella avium and Bacillus megaterium in Endodontic Infection. Colloid Surface B. against Aedes aegypti (Diptera: Culicidae) - the vector of Chikungunya and Dengue. (1983). Approved list of bacterial names. 1978). No hemolytic activity was observed in B. megaterium strain ATCC 14581 by Health Canada scientists. This strain was added to the DSL under subsection 25(1) of CEPA 1988 and the DSL under subsection 105(1) of CEPA because it was manufactured in or imported into Canada between January 1, 1984 and December 31, 1986. Plant Dis 96, 211-220.
Bertone, C., Michalak, P., and Roda, A. Further details on the risk assessment methodology used are available in the Risk Assessment Framework document “Framework on the Science-Based Risk Assessment of Micro-organisms under the Canadian Environmental Protection Act, 1999” (Environment Canada and Health Canada 2011). J Geotech Geoenviron Eng 140. Identification of Bacillus Species. For methods and additional details, see MIDI labs. Ercolani, G. (1978). Human exposure to B. megaterium strain ATCC 14581 is expected to be medium based on the wide range of uses reported in the section 71 survey. constitute or may constitute a danger in Canada to human life or health. Shark, K.B., Smith, F.D., Harpending, P.R., Rasmussen, J.L., and Sanford, J.C. (1991). Growth on nutrient agar after 24 hours at 30oCa, Growth on nutrient agar after 48 hours at 30oCa, Growth on TSBb agar after 24 hours at 37oCa, Growth on TSB agar after 48 hours at 37oCa, Growth on ATCC Medium 3 (nutrient agar), 24 hours at 30oCc, a Data generated by Environmental Health Science and Research Bureau, Health Canada. (Arya et al. 2011). Health Canada scientists measured the optical density of 24-hour B. megaterium strain ATCC 14581 cultures in various liquid media grown at various temperatures. Production of natural methyl anthranilate by microbial N-demethylation of N-methyl methyl anthranilate by the topsoil-isolated bacterium Bacillus megaterium. Achal, V., and Pan, X. No statistically significant difference between treatments was observed in mean adult survival or mean juvenile production after 28 days following the initial inoculation event. 1991). (2005). Evaluation of larvicidal activity of soil microbial isolates (Bacillus and Acinetobactor Sp.) (1985). Spores of B. megaterium strain ATCC 14581 are likely to persist and could accumulate in the environment. Effects of Freezing on the Survival of Escherichia coli and Bacillus and Response to UV and Chlorine After Freezing. Three hypotheses of the epithet "megaterium" are possible:, Consequently, it was decided in the first juridical opinion of the Bacteriological code that the name should remain "megaterium" given the unclear meaning. 2002; Barbosa et al. CFIA. 2007). Information submitted in response to the section 71 notice indicates that 10 000 to 100 000 kg of products containing B. megaterium strain ATCC 14581 were imported into or manufactured in Canada in 2008. Identification and application of AFLP-derived genetic markers for quantitative PCR-based tracking of Bacillus and Paenibacillus spp. 2014; Rosso and Vary 2005; Vary et al. An in-depth review of the scientific literature did not identify adverse effects of B. megaterium or B. megaterium strain ATCC 14581 in aquatic plant species. Annotations from the NCTC on the List of Type and Reference Strains of Bacteria, Sneath and Skerman 1966. (2010). Identification and characterization of clinical Bacillus spp.
2014) using a variety of search strategies (nucleotide and protein searches) and did not find any major virulence factor genes. (1997). 2011); Meloidogyne graminicola (Padgham and Sikora 2007) and Meloidogyne incognita (Saikia et al.
Euzéby, J.P. and Tindall, B.J.  This trend continues as many scientists (mainly from the developing world) still use the name B. megatherium, sowing confusion. (2014). BAuA. Spores of Bacillus species can withstand temperatures about 45°C higher than vegetative cells (Coleman et al. Liu, L., Li, Y., Zhang, J., Zou, W., Zhou, Z., Liu, J., Li, X., Wang, L., and Chen, J. (1988). Appl Environ Microbiol 78, 8470-8473.
2010) and humans (Weber et al. Biochemistry (N. Technical guidance: Update of the criteria used in the assessment of bacterial resistance to antibiotics of human n or veterinary importance.
2003; West et al. Isolation and characterization of PGPR and their effect on growth, yield and nutrient content in wheat (Triticum aestivum L.). Health Hazard Evaluation Report. B. megaterium strain ATCC 14581 was observed to grow well in TSB at 28°C but limited growth was observed in serum-containing mammalian culture media at this temperature. B. megaterium as a species is generally considered ubiquitous and is found in a variety of habitats, including: Strains of B. megaterium, including ATCC 14581, grow over a wide range of temperatures, with minimum growth temperatures between 3°C and 10°C, an optimal growth temperature of 37°C, and a maximum growth temperature of 40 to 45°C. (2004). 2013).
Larsen, N., Thorsen, L., Kpikpi, E.N., Stuer-Lauridsen, B., Cantor, M.D., Nielsen, B., Brockmann, E., Derkx, P.M., and Jespersen, L. (2014). Lipid and protein composition of membranes of Bacillus megaterium variants in the temperature range 5 to 70 degrees C. J Bacteriol 135, 1043-1052. 1979), paper linerboard (Namjoshi et al. (2005).
Efficacy of Using Harmless Bacillus Endospores to Estimate the Inactivation of Cryptosporidium parvum Oocysts in Water. 2010) and as contaminants of food (Kurtzman et al. Garvey, M., Clifford, E., O'Reilly, E., and Rowan, N.J. (2013). 2006). Sadiq, A., and Ali, B. Soil Biol Biochem 25, 849-855. (2012).
(S.C. 2009, c. 24). 2001). released in soil.
No effects in aquatic plants, invertebrates or vertebrates or terrestrial vertebrates have been reported in the scientific literature. Plasmid 53, 205-217. Sandeep, C., Raman, R.V., Radhika, M., Thejas, M.S., Patra, S., Gowda, T., Suresh, C.K., and Mulla, S.R. 51, 46-52. White blotch incited in wheat by Bacillus megaterium pv. 2013; Verma et al.